FIGURE 4.
MAG-induced β1-integrin internalization and inhibition of clustering is PTEN-dependent. A, representative immunofluorescence images show the distribution of β1-integrin in the growth cone of wild-type and PTEN-MO neurons after experimental treatments: control (BSA), MAG (1 μg/ml; 5 min), or (Sp)-cAMPS (20 μm) + MAG. Arrowheads designate clustered β1-integrin. Scale bar, 5 μm. B and C, summary graphs show the quantification of β1-integrin surface levels (B) and β1-integrin clustering (C) for all experimental conditions. Data are the mean ± S.E. (n > 150, *, p < 0.05, one-way ANOVA with a Tukey's post hoc analysis).