FIGURE 5.

The rescue effect of carbamazepine is not due to activation of autophagy. A, Western blot of SUR1 from COSm6 cells transfected with F27S SUR1 and WT Kir6.2 and treated with carbamazepine (CBZ), chloroquine (Chloroq), or both for 16 h (upper panel) or carbamazepine, Ly294002, or both for 16 h (lower panel) as indicated. WT SUR1 is shown as a control. B, Western blot of SUR1 from COSm6 cells transfected with F27S SUR1 and WT Kir6.2 and treated with carbamazepine or rapamycin (Rapa) or Li⁺, both of which are autophagy inducers, for 16 h as indicated.