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Localization of PRMT5, H4R3me2s modification, and DNMT3A on C/EBPβ target genes. Occupancies on the promoter (P) and exon (E) regions of IL-8 (A) and TNFα (B) genes were measured by ChIP followed by qPCR. PMA treatment was performed at 25 ng/ml for 2 h. Immunoprecipitations were carried out individually three times (n = 3). The data were normalized by defining the signal level in the absence of PMA treatment as 1. The error bars show S.D. *, p < 0.05; **, p < 0.01.
