FIGURE 2.

mTORC1 contributes to autophagy suppression after feeding. A, mice were starved for 24 h (starved) and then refed for 1 h (refed). Rapamycin (0.4 mg/kg), a specific inhibitor of mTORC1, or saline with 5% Tween 20 and 10% DMSO (vehicle) was intraperitoneally injected at 2 h and 16 h before tissue sampling. Muscle and liver samples were subjected to immunoblot analysis using the indicated antibodies. B, quantitative analysis of the immunoblot data in skeletal muscle and liver samples using densitometry scanning. The LC3-II/total LC3 (LC3-I+II) ratio were calculated. The anti-LC3 antibody used in this study reacts to both LC3-I and LC3-II with similar affinities (supplemental Fig. S2). Data are mean ± S.E. of 4 mice (two are shown in A and the other two are not shown). *, p < 0.05; **, p < 0.01; n.s., not significant.