FIGURE 2.

ICI activates AF2ER-dependent, ERE-mediated transcription. HepG2 cells were cotransfected with the reporter gene (3xERE-TATA-luc), reference gene (pRL-TK), and expression vector for WT ERα (A); N-terminal truncated ERα (121-ERα) (B); AF2ER (C); or N-terminal truncated AF2ER (121-AF2ER) (D) and treated with either vehicle (0 nm), E2 (0.01–10 nm, ●), or ICI182780 (0.01–10 nm, ○). The luciferase activities are represented as fold change over vehicle (0 nm). Luciferase activity is represented as mean ± S.D. a, p < 0.001 against vehicle (0 nm) in each panel. E, Whole cell lysates extracted from vehicle-treated (V), E2-treated (1 nm), and ICI-treated (10 nm) transfected HepG2 cells were analyzed by immunoblotting with the anti-ERα antibody (MC-20, ERα). β-actin (Actin) was used as a loading control. A representative Western blot analysis is shown.