Figure 4.

Two-color FACS analysis of B cells with anti-Gal BCR in α1,3GT KO mice. B cells were identified by the staining with PE-conjugated anti-mouse Ig, whereas cells with anti-Gal BCR were identified by the binding of biotinylated α-gal BSA, followed by avidin-FITC. The stained lymphocytes were from: (a) nonimmunized mice; (b) mice immunized with pig kidney membranes; (c) cells from mice immunized as in b and tested for binding of biotinylated N-acetyllactosamine (Galβ1-4GlcNAc-R)-BSA as specificity control; (d) mice immunized with WT mouse kidney membranes; (e) mice immunized with glycolipids from rabbit RBC. Proportion of double-positive stained cells is indicated. Data are from a representative mouse of 5 in each group.