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. 2000 Feb 1;105(3):329–339. doi: 10.1172/JCI7398

Figure 7.

Figure 7

(a) Effect of colchicine on surface Fas density of SECs. SECs were isolated from liver 24 hours after mice were pretreated in vivo with colchicine or γ-lumicolchicine. The cells were stained with 2 mg/mL PE-labeled Jo2 antibody and were assessed for surface Fas density by FACS® analysis as described in Methods. Solid peaks are unstained controls; open peaks are stained pretreated cells. Left: SEC from γ-lumicolchicine-pretreated mice. Right: SEC from colchicine-pretreated mice. (b) Effect of colchicine on Jo2-induced apoptosis of SECs. SECs were pretreated with 1 μM colchicine or γ-lumicolchicine for 12 hours and then were exposed to 100 ng/mL Jo2 antibody for 8 hours. The treatments are indicated below each bar. Results are the mean ± SD for 3 independent experiments.