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. 2000 Feb 1;105(3):311–320. doi: 10.1172/JCI7535

Figure 4.

Figure 4

Effect of exercise on insulin stimulation of ERK phosphorylation (a), ERK activity (b), and MEK activity (c). Obese (n = 6) and diabetic (n = 6) subjects underwent euglycemic clamps with muscle biopsies twice: 1 time without prior exercise and again 24 hours after a single 1-hour bout of moderate exercise. Homogenates of muscle biopsy specimens were analyzed for ERK phosphorylation by immunoblot analysis using an antiphosphoERK antibody. ERK activity was determined as described in the text using anti-ERK immunoprecipitates, and MEK activity was assayed using anti-MEK immunoprecipitates. Basal values are shown as open bars, and insulin-stimulated values are shown as filled bars. Data are presented as means ± SEM. *P < 0.05 versus basal values. †P < 0.05 versus lean control values.