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Binding of farnesyl-Cys-AMCA to AIPL1 and its mutants
| AIPL1 mutation | Kd |
|---|---|
| μm | |
| Wild type | 0.11 ± 0.01a |
| R38C | 0.15 ± 0.03 |
| K53W | 0.15 ± 0.02 |
| P58A | 0.17 ± 0.02 |
| M59A | 0.11 ± 0.01 |
| V71F | 0.19 ± 0.06 |
| W72S | 0.10 ± 0.01 |
| V96I | 0.13 ± 0.01 |
| T114I | 0.12 ± 0.01 |
| Y134F | 0.26 ± 0.01 |
| V148A | 0.21 ± 0.05 |
| F149A | 0.21 ± 0.06 |
| L150A | 0.13 ± 0.02 |
| L154A | 0.19 ± 0.01 |
a Mean ± S.E. (n ≥ 3).
