FIGURE 9.

Abundance of DrRecA-cssDNA filaments increases with time in the presence of DrSSB. DrRecA was incubated with M13mp18 ldsDNA and ATP for 15 min before ΦX174 cssDNA was added for 5 min followed by DrSSB. The reaction was stopped at 0, 5, 10, and 20 min after the addition of DrSSB, fixed with ATPγS, and spread on Alcian grids. A, bar graph shows the decrease in long linear filaments with time, and increase in circular filaments, especially big circular filaments. B, at least four individual molecules were measured in each class of DrRecA filament in eight samples (duplicates of each time point). Diamonds represent molecules from 0-min samples, squares from 5-min samples, triangles from 10-min samples, and circles from 20-min samples. Long linear molecules range between 2.7 and 7.4 μm and short linear range between 0.2 and 1.1 μm. Small circles range between 0.1 and 1.3 μm; medium circles are between 0.8 and 2.1 μm, and large circles typically range between 2.1 and 3.7 μm. No small circles with small gaps were observed at the 10- and 20-min time point samples.