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. 2013 Jun 6;288(29):21376–21388. doi: 10.1074/jbc.M113.491514

FIGURE 1.

FIGURE 1.

Kdm4b- and KDM4D-EGFP recruit to DNA damage. U2OS osteosarcoma cells were transfected with Kdm4b-EGFP. 24 h after transfection 2-photon laser micro-irradiation was used to measure the recruitment of EGFP-tagged Kdm4 demethylases to DNA damage. A, both Kdm4b-EGFP and KDM4D-EGFP recruited readily to the DNA damage tracks. Graphs represent the mean values for 18, 30, 25, and 16 nuclei ± S.E. (Kdm4a–c, respectively). B, Kdm4a-EGFP also recruited to the DNA damage tracks but at later time points. C, representative images show the accumulation of Kdm4b-EGFP (C, top) and KDM4D-EGFP (C, bottom) on the DNA damage tracks.