FIGURE 5.

Kdm4-EGFP does not accumulate at IRIF. U2OS cells were plated on glass coverslips and transfected with EGFP-tagged Kdm4a (A), Kdm4b (B), Kdm4c (C), or KDM4D (D). 24 h after transfection, cells were treated with 2 Gy of γ-irradiation and fixed at 60 min post-irradiation. Cells were stained for γH2AX, and the nuclei were counterstained with DAPI. Line scans show the fluorescence intensity for the EGFP-tagged Kdm4 protein (gray line, curve area filled) and the Cy3-stained γH2AX (black line). The line scans shown were performed on the lines indicated in the Kdm4-EGFP panels (delineated by arrows).