FIGURE 3.

Various Hsp110s and Hsp40s can collaborate to reactivate various misfolded protein substrates. A, time-dependent refolding of thermally predenatured G6PDH following 30 min of incubation at 30 °C without or with ATP, 6 μm Hsp70 or Hsp110 (HSPH1), without or with 3 μm DNAJA1, as indicated. B, refolding yields of urea-preunfolded MDH (600 nm final concentration) at 34 °C. 60 μm MDH was incubated 30 min at 34 °C in 8 m urea and diluted 100-fold in refolding buffer also containing 5% glycerol, without or with 4 μm Hsp70, or Hsp110 (Hsp105) and 2 μm DNAJA1, as indicated. C, time-dependent refolding of 1 μm FTluc without or with ATP, 1 μm Apg-2 or Hsp105, and 0.5 μm DNAJA1 or DNAJB1. The protein combinations are indicated.