TABLE 1.
Rate of ATP hydrolysis and ATP cost by various combinations of Hsp110, Hsp70, and Hsp40, without or with equimolar aggregated (Agg.) or misfolded monomeric (Mono.) luciferase (Luc.) substrates
| Sample | ATP consumed by 500 nm chaperones only | ATP consumed by chaperones in the presence of substrates | ATP | Net ATP cost to refold a luciferase |
|---|---|---|---|---|
| min−1 | min−1 | chaperone−1 min−1 | ||
| Hsp70 | 312 | 0.62 | ||
| Hsp110 | 252 | 0.50 | ||
| Hsp70 + Hsp40 | 624 | 1.25 | ||
| Hsp110 + Hsp40 | 516 | 1.03 | ||
| Hsp70 + Hsp110 + Hsp40 | 924 | 1.85 | ||
| Hsp70 + Hsp40 + Luc. Mono. | 1392 | 2.78 | 77.45 | |
| Hsp110 + Hsp40 + Luc. Mono. | 1644 | 3.29 | 168.6 | |
| Hsp70 + Hsp110 + Hsp40 + Luc. Mono | 2772 | 5.54 | 148.9 | |
| Hsp70 + Hsp40 + Luc. Agg. | 1536 | 3.07 | 650.7 | |
| Hsp110 + Hsp40 + Luc. Agg. | 1890 | 3.8 | 485.4 | |
| Hsp70 + Hsp110 + Hsp40 + Luc. Agg. | 2388 | 4.8 | 228.9 |