FIGURE 10.

Predicted biosynthetic routes for core N-glycan modifications in nematodes. Tested biosynthetic routes (solid lines) involved in the formation of the core chitobiose modifications are summarized based on our experimental data, including reactions of pyridylaminated sugars, dabsylated glycopeptides, and chemically synthesized compounds; predicted GO or NO-GO reactions (broken lines) are judged on substrate specificities of relevant glycoenzymes; short broken lines with a double bar are “dead ends.” FT, fucosyltransferase; Hex, jack bean hexosaminidase; GT1, galactosyltransferase GALT-1. Red triangles, fucose; yellow circles, galactose; blue squares, N-acetylglucosamine; green circles, mannose.