FIGURE 4.
Glycomic data of double fucosyltransferase mutants. Peptide:N-glycanase F (PNGase F)-released glycan pools of fut-1;fut-6 (F1F6), fut-1;fut-8 (F1F8), and fut-6;fut-8 (F6F8) mutants were examined by MALDI-TOF MS. Peptide:N-glycanase A digestion, after peptide:N-glycanase F treatment, of F1F6 and F1F8 resulted in only trace amounts of the same structures. Glycan compositions are abbreviated in the form HxNyFz (i.e. HexxHexNAcyFucz).