Fig. 8.

IκB-αM prevents TGF-β-induced cell motility in pancreatic cancer cells. Cells were transfected with the mutant IκB-αM for 24 h. The cells were then seeded on the Boyden chambers, incubated with TGF-β for 4 h, then fixed in methanol. The fixed cells on the bottom of the membrane in each chamber were stained with Giemsa stain and observed under the microscope. Cells in 5 fields were recorded, and the number of cells that cross the membrane were counted and averaged. TGF-β induced cell motility, and transfection with the mutant IκB-αM reversed the number of cells that cross the membrane after TGF-β treatment. *P < 0.05, ***P < 0.001 vs. Control; +++P < 0.001 vs. TGF-β. Data were means ± SE of 3 samples.