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. 2012 Nov 30;304(3):H415–H426. doi: 10.1152/ajpheart.00468.2012

Fig. 7.

Fig. 7.

Nampt regulates cardiac fibroblast proliferation, differentiation, and apoptosis. A: primary cultures of cardiac fibroblasts were treated with 100 ng/ml Nampt or infected with Ad.Nampt, and cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay after 48 h of plating. Means ± SD; n = 5. B: primary cultures of cardiac fibroblasts were treated with 100 ng/ml recombinant eNampt or infected with 10 MOI of Ad.Nampt. Cells were immunostained for collagen 1 (green) and α-SMA (red). C: primary cultures of cardiac fibroblasts were treated with 100 ng/ml recombinant eNampt or infected with 10 MOI of Ad.Nampt. Cell lysates were analyzed by Western blot analysis with antibodies as indicated. D: primary cultures of cardiac fibroblasts were treated with cell death-inducing dose (500 μM) of H2O2 in the presence or absence of recombinant Nampt for 1 h. Extent of apoptosis was measured by estimating the percentage of annexin V-positive cells by fluorescent-activated cell-sorting analysis. E: results of 4 independent experiments. Means ± SD; n = 4.