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Inhibition of [3H]SQ29548 specific binding to mouse platelets by U46619 (■), 12(R)-HETE (▲), and 12(S)-HETE (●). Specific binding (total binding − nonspecific binding) was normalized to 100% of maximum binding. IC50 were 0.07, 0.32, and 1.73 μM for U46619, 12(R)-HETE, and 12(S)-HETE, respectively. Data were analyzed using the nonlinear regression analysis of PRISM (GraphPad). Each value represents the mean ± SE; n = 3.
