Table 2.

Antibodies used

	Origin	Used to Visualize	Company	Catalog Number	Dilution	Incubation
Primary antibodies
NCAM	Mouse	Satellite cells	Becton Dickinson (San Jose, CA)	347740	1:100 and 1:50	4°C overnight
Ki67	Rabbit	Active cells	Biocare Medical (Concord, CA)	CP249	1:200	2 h at room temperature
Pax7	Mouse	Satellite cells	Neuromics (Edina, MN)	MO15020	1:100	4°C overnight
Laminin	Rabbit	Basal lamina	Dako Norden (Glostrup, Denmark)	Z0097	1:200	4°C overnight
CD16	Mouse	Neutrophils	Dako Norden	M7006	1:200	4°C overnight
CD68	Mouse	Macrophages	Dako Norden	M0718	1:200	4°C overnight
Desmin	Mouse	Desmin-negative fibers	Zymed (San Francisco, CA)	18-0016	1:50	4°C overnight
Myosin heavy chain (developmental)	Mouse	Embryonic myosin	Developmental Studies Hybridoma Bank (Iowa City, Iowa)	FI.652	1:50	4°C overnight
Secondary antibodies
Anti-mouse biotinylated	Goat	Mouse primary antibodies	Dako Norden	E0433	1:200	45 min at room temperature
Immunoflourescent secondary antibodies
Alexa fluor 568 anti-mouse red	Goat	Mouse primary antibodies	Molecular Probes, Invitrogen (Taastrup, Denmark)	A11031	1:100 and 1:200	45 min at room temperature
Alexa fluor 488 anti-rabbit green	Goat	Rabbit primary antibodies	Molecular Probes, Invitrogen	A11034	1:100 and 1:200	45 min at room temperature