| Reagent | Volume (μl) |
|---|---|
| Eluted DNA | 7.5 |
| 10X Blunt Buffer | 1.25 |
| 1 mM dNTP mix | 1.25 |
| Blunt Enzyme Mix | 0.5 |
| E. coli DNA ligase for Fragmentase | 0.4 |
| Sterile dH2O | 1.6 |
|
| |
| Final Volume | 12.5 |
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