| Reagent | Volume (μl) |
|---|---|
| Eluted DNA | 6 |
| 2X Quick Ligation Buffer | 7.5 |
| Barcoded Adaptor (45 μM) | 1.2 |
| Quick T4 DNA Ligase | 0.6 |
|
| |
| Final Volume | 15.3 |
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