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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cold Spring Harb Protoc. 2013 Sep 1;2013(9):10.1101/pdb.prot074187 pdb.prot074187. doi: 10.1101/pdb.prot074187
Reagent Volume (μl)
5X Phusion Buffer 5
Multi/Std PCR Primer 1.0 (25 μM) 0.5
Multiplexing PCR Primer 2.0 (0.5 μM) 0.5
PCR Primer Index 1 (or others) (25 μM) 0.5
dNTP mix (10 mM) 0.75
Phusion DNA polymerase 0.5
Gel Extracted Sample 7.5
Sterile dH2O 9.75
Final Volume 25