| Reagent | Volume (μl) |
|---|---|
| 5X Phusion Buffer | 10 |
| Multi/Std PCR Primer 1.0 (25 μM) | 1 |
| Multiplexing PCR Primer 2.0 (0.5 μM) | 1 |
| PCR Primer Index 1 (or others) (25 μM) | 1 |
| dNTP mix (10 mM) | 1.5 |
| Phusion DNA polymerase | 1 |
| Gel Extracted Sample | 15 |
| Sterile dH2O | 19.5 |
|
| |
| Final Volume | 50 |
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