Table 3A.
Procedure B - 100 Samples
| Reagent | Total Samples | Company | Total Preps | Catalog # | Quantity | Price | Total Price | Per Unit Cost |
|---|---|---|---|---|---|---|---|---|
| Fragmentase | 100 | NEB | 100 | M0348S | 1 | 96 | 96 | 0.96 |
| ZR-96 Pur Kit | 100 | Zymo | 4×96 | D4024 | 1 | 387 | 387 | 3.87 |
| Quick Blunt | 100 | NEB | 120 | E1201S | 3 | 77 | 231 | 1.92 |
| Klenow exo- | 100 | NEB | 133.3 | M0212L | 1 | 228 | 228 | 1.71 |
| Quick Ligation | 100 | NEB | 100 | M2200S | 2 | 95 | 190 | 1.9 |
| Phusion | 100 | NEB | 50 | M0530S | 1 | 103 | 103 | 2.06 |
| Phusion-Pool 8 | 0.25 | |||||||
| Phusion-Pool 12 | 0.17 | |||||||
| Pool of 8 | Pool of 12 | |||||||
| Enzyme Cost: | 6.75 | 6.66 | ||||||
| Total Cost: | 1235 | Column Cost: | 3.87 | 3.87 | ||||
| Per Sample: | 12.35 | Total Unit Cost Per Sample: | 10.62* | 10.53* |
*
The cost of gel extraction columns and polymerase costs are not included in the cost estimation of Procedure B because the enzymatic unit cost is negligible when applied across 96 or more samples.