Table 3B.
Procedure B - 200 Samples
| Reagent | Total Samples | Company | Total Preps | Catalog # | Quantity | Price | Total Price | Per Unit Cost |
|---|---|---|---|---|---|---|---|---|
| Fragmentase | 200 | NEB | 200 | M0348S | 2 | 96 | 192 | 0.96 |
| ZR-96 Pur Kit | 200 | Zymo | 4×96 | D4024 | 1 | 387 | 387 | |
| ZR-96 Pur Kit | 200 | Zymo | 2×96 | D4023 | 1 | 199 | 199 | 2.93 |
| Quick Blunt | 200 | NEB | 200 | E1201L | 1 | 308 | 308 | 1.54 |
| Klenow exo- | 200 | NEB | 266.6 | M0212L | 2 | 228 | 456 | 1.26 |
| Quick Ligation | 200 | NEB | 250 | M2200L | 1 | 380 | 380 | 1.71 |
| Phusion | 200 | NEB | 50 | M0530S | 1 | 103 | 103 | 2.06 |
| Phusion-Pool 8 | 0.25 | |||||||
| Phusion-Pool 12 | 0.17 | |||||||
| Pool of 8 | Pool of 12 | |||||||
| Enzyme Cost: | 5.7345 | 5.64 | ||||||
| Total Cost: | 2025 | Column Cost: | 2.93 | 2.93 | ||||
| Per Sample: | 10.12 | Total Unit Cost Per Sample: | 8.66* | 8.57* |
*
The cost of gel extraction columns and polymerase costs are not included in the cost estimation of Procedure B because the enzymatic unit cost is negligible when applied across 96 or more samples.