Table 3C.
Procedure B - 400 Samples
| Reagent | Total Samples | Company | Total Preps | Catalog # | Quantity | Price | Total Price | Per Unit Cost |
|---|---|---|---|---|---|---|---|---|
| Fragmentase | 400 | NEB | 500 | M0348L | 1 | 384 | 384 | 0.768 |
| ZR-96 Pur Kit | 400 | Zymo | 4×96 | D4024 | 3 | 387 | 1161 | 2.90 |
| Quick Blunt | 400 | NEB | 400 | E1201L | 2 | 308 | 616 | 1.54 |
| Klenow exo- | 400 | NEB | 399.9 | M0212L | 3 | 228 | 684 | 1.71 |
| Quick Ligation | 400 | NEB | 500 | M2200L | 2 | 380 | 760 | 1.52 |
| Phusion | 400 | NEB | 50 | M0530S | 1 | 103 | 103 | 2.06 |
| Phusion-Pool 8 | 0.12 | |||||||
| Phusion-Pool 12 | 0.08 | |||||||
| Pool of 8 | Pool of 12 | |||||||
| Enzyme Cost: | 5.66 | 5.62 | ||||||
| Total Cost: | 3708 | Column Cost: | 2.90 | 2.90 | ||||
| Per Sample: | 9.27 | Total Unit Cost Per Sample: | 8.56* | 8.52* |
*
The cost of gel extraction columns and polymerase costs are not included in the cost estimation of Procedure B because the enzymatic unit cost is negligible when applied across 96 or more samples.