Fig. 2. Defective ubiquitination and mitophagy in Mfn2-deficient mouse hearts.
(A) Mfn2 ubiquitination in PINK1/Parkin-containing HEK cell immune complexes (left). Input proteins are on right. (B) Protein ubiquitination stimulated by FCCP in Mfn2/Parkin immune complexes from mouse heart. (C) Mitochondrial ubiquitination (green) induced by FCCP in wild-type, Mfn1-, and Mfn2-deficient mouse cardiomyocytes. Cell borders are outlined in white. (D) Parallel studies of mitophagy-adaptor protein p62/SQSTM1 (green).