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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Immunol. 2013 Jul 12;191(4):2009–2017. doi: 10.4049/jimmunol.1301317

Figure 1. Immune complexity of human NSCLC.

Figure 1

(A) Hematoxylin and eosin (H&E) staining of human non-small cell lung cancer (NSCLC) and adjacent normal tissue (top panel) with representative images showing staining for CD45 (bottom panel). (B) Numbers of CD45+ leukocytes per square millimeter of tissue sections as assessed immunohistochemistry. n=8 samples per group. (C) Flow cytometric analysis of immune cell infiltrates within human NSCLC represented as percentage of total CD45+ leukocytes. n=6 samples per group. (D) CD19+CD20+HLA-DR+ B cell and CD3+CD4+ T cell infiltrate within human NSCLC as assessed by flow cytometry, shown as a percent of total CD45+ cells. (E) Percent of CD4+ and CD8+ T cells staining positive for CD69 as assessed by flow cytometry, with representative histograms of CD69 expression shown to the right. *p<0.05; **p<0.01, ***p<0.001.

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