Figure 5. CD8⁺ T cells in NSCLC following T_reg depletion.

(A) Frequency of BrdU⁺ proliferating CD8⁺ T cells represented as percentages of total tumor infiltrating CD8⁺ T cells. (B) Frequency of CD69-expressing activated CD8⁺ T cells represented as percentages of total tumor infiltrating CD8+ T cells. (A-B) n=7 per group, one of two representative experiments is shown. (C-F) Relative expression of Ifng (C), Gzma (D) Gzmb (E) and Prf1 (F) mRNA in flow-sorted CD8⁺ T cells represented as fold change over Tbp as assessed by qPCR. n=7 per group, with data obtained over 2 independent cohorts of animals. (G) Tumor burden represented as percentage of lung area, following treatment with αCD25 mAb from 4 weeks until 8 weeks of age in CC10-TAg mice deficient in CD8⁺ T cells. n=10 per group, with data obtained over 3 independent cohorts of animals. *p<0.05; **p<0.01, ***p<0.001.