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. 2000 Mar 15;105(6):757–764. doi: 10.1172/JCI6816

Figure 3.

Figure 3

Co-immunoprecipitation of ALAS-E and SCA-βA. (a) Schematic representation of FLAG-tagged ALAS-E expression vector and HSV-6xHis–tagged SCS-βA expression vector. (b) Coimmunoprecipitation analysis of ALAS-E and SCS-βA. Full-length human ALAS-E and human SCS-βA were expressed as FLAG epitope–tagged and HSV-6xHis epitope–tagged proteins, respectively. After immunoprecipitation with anti-FLAG monoclonal antibody (lanes 1–4 and 9–12) or anti-6xHis polyclonal antibody (lanes 5–8 and 13–16), the immune complex was examined by Western blot analysis, using as the primary antibody either anti-FLAG monoclonal antibody (lanes 1–8), anti-6xHis polyclonal antibody (lanes 9–12), or anti-HSV monoclonal antibody (lanes 13–16). p, precursor protein; m, mature protein; IP, antibody used for immunoprecipitation.