Fig. 2.

Immunolocalization of rSGLT2 and cell adhesion molecule CAM105 in proximal tubular segments. Staining with rSGLT2-Ab (A, C) and CAM105-Ab (B, D) was performed in consecutive sections of rat kidney cortex (A, B) and outer stripe (C, D). In cortex (A), rSGLT2-Ab stained the high brush border of S1 segments and the low brush border of S2 segments. In accordance with previous data (37), these segments can be distinguished by BBM staining with CAM105-Ab, which is strong in the S1 segment (B, arrowheads) and weak or absent in the S2 segment (B). In the outer stripe, the brush border of S3 segments was negative for rSGLT2 (C) and strongly positive for CAM105 (D, arrowheads). Data represent findings in 2 female rats. Cell membranes of the glomerular (G) and peritubular capillaries (arrows, B and D) were also positive for CAM105. Bar, 20 μm.