Fig. 3.

SDS-PAGE and Western blot analysis of proteins in the mitochondrial subfractions. A: silver nitrate-stained protein bands after running the mitochondrial subfractions (1–9) on a 4–15% gradient gel. The lane labeled MW was loaded with molecular weight markers. *Predicted bands for hexokinase (HK); **predicted bands for voltage-dependent anion channel (VDAC) with the adenine nucleotide translocator of IMM (ANT). B: representative Western blots of VDAC (OMM marker), prohibitin (IMM marker), respiratory complex III (CIII; IMM marker), and calsequestrin (CSQ; SR marker). RHM, rat heart mitochondrial fraction.