Fig. 6.
A: protocol to determine whether a VLC or 15 min of RC causes regeneration of APS. After the determination of Lref (Fig. 1B), 3 pairs of Tp measurements were made at Lref (shaded bars) while in 0 Ca, as in the previous protocols (Figs. 3A and 5A). The first pair measured the amount of Tp restored by two 1-min KCl-induced contractions at 50% Lref (points 4 and 5) following 7 strain-softening stretches to 133% Lref (open bars). The second pair of Tp measurements (points 8 and 10) measured the amount of Tp restored by a VLC (point 8, arrow), and the third pair measured the amount of Tp restored by 15 min of PGE2-induced RC at 50% Lref (point 13). B: Tp increase at Lref due to 2 contractions, VLC, or 15-min RC induced with PGE2. Data were normalized to the increase due to 2 contractions. Values are means ± SE (n = 4). *P < 0.05 indicates a significant difference compared with 1.0.