Fig. 1.
Western blots demonstrate permanent knockout of promyostatin within 2 wk after administration of tamoxifen (TMX) to Mstnf/f mice (f). Age-matched Mstnw/w mice (w) received the same regimen of tamoxifen to induce Cre recombinase activity. Top 2 blots were done with muscle protein samples in which nonglycosylated proteins were removed with concanavalin A (2). Samples in bottom blot were not processed with concanavalin A. Samples used for top and bottom blot were not reduced, and bands are at expected location for propeptide dimer. Samples used for middle blot were reduced, and bands are at expected location for propeptide monomer. Memcode staining was done to confirm even loading and transfer of proteins. Locations of prestained standards (Benchmark, Invitrogen) are denoted by the corresponding molecular weights (kDa).