Table 1.
Differentially expressed genes in Lasp1−/− mouse embryonic fibroblasts categorized by recognized functions
| Gene Symbol | Description | Accession Number | Fold Change(s) | P Value(s) | ||||
|---|---|---|---|---|---|---|---|---|
| Cell motility/migration | ||||||||
| Acta1* | actin, α1, skeletal muscle | NM_009606 | 2.870 | 0.015 | ||||
| Lpp† | LIM domain containing preferred translocation partner in lipoma | AK045341 | 2.24; 2.262; 2.298 | 0.039; 0.038; 0.002 | ||||
| Slit3‡ | slit homolog 3 (Drosophila) | XM_203363 | −2.985 | 0.002 | ||||
| Tnn‡ | tenascin N (tenascin-W) | NM_177839 | −2.316 | 0.000 | ||||
| Flt1‡ | FMS-like tyrosine kinase 1 | NM_010228 | −2.128 | 0.035 | ||||
| Cell adhesion/extracellular matrix organization and biogenesis | ||||||||
| Col2a1† | procollagen, type II, α1 | NM_031163 | 6.674; 3.020; 5.171 | 0.000; 0.000; 0.002 | ||||
| Nrp | neuropilin 1 | AK030358 | 2.454 | 0.039 | ||||
| Nid1 | nidogen 1 | AK049585 | 2.435 | 0.026 | ||||
| Klra4 | killer cell lectin-like receptor, subfamily A, member 4 | NM_010649 | 2.228 | 0.002 | ||||
| Col10a1 | procollagen, type X, α1 | NM_009925 | 2.181 | 0.005 | ||||
| Matn4 | matrilin 4 | NM_013592 | −3.356 | 0.039 | ||||
| Mfap4 | microfibrillar-associated protein 4 | NM_029568 | −2.725 | 0.040 | ||||
| Cdh3 | cadherin 3 | NM_007665 | −2.231 | 0.007 | ||||
| Dpt | dermatopontin | NM_019759 | −2.179 | 0.002 | ||||
| Comp | cartilage oligomeric matrix protein | NM_016685 | −2.075 | 0.044 | ||||
| Cytoskeleton organization and biogenesis | ||||||||
| Msn‡ | moesin | AK031171 | 2.026 | 0.022 | ||||
| Krt1-14 | keratin 14 | NM_016958 | −6.024 | 0.003 | ||||
| Fgf21 | fibroblast growth factor 21 | NM_020013 | −2.519 | 0.008 | ||||
| Regulation of cell proliferation through cell cycle | ||||||||
| Cxcl1 | chemokine (C-X-C motif) ligand 1 | NM_008176 | 3.199 | 0.001 | ||||
| Fgf7 | fibroblast growth factor 7 | NM_008008 | 2.430 | 0.001 | ||||
| Bcl11b | B-cell leukemia/lymphoma 11B | NM_021399 | −3.745 | 0.009 | ||||
| Osr2† | odd-skipped related 2 (Drosophila) | NM_054049 | −2.519; −2.331 | 0.013; 0.005 | ||||
| Cell growth | ||||||||
| Socs2 | suppressor of cytokine signaling 2 | NM_007706 | −2.346 | 0.007 | ||||
| Igfbp3‡ | insulin-like growth factor binding protein 3 | NM_008343 | −2.124 | 0.015 | ||||
| Htra1† | HtrA serine peptidase 1 | NM_019564 | −2.062; −2.016 | 0.001; 0.002 | ||||
| Apoptosis | ||||||||
| Phlda1 | pleckstrin homology-like domain, family A, member 1 | NM_009344 | 2.206 | 0.001 | ||||
| Cd40† | CD40 antigen | NM_170702 | −2.160; −2.387 | 0.001; 0.000 | ||||
| Iron ion transport | ||||||||
| Cp | ceruloplasmin | NM_007752 | 2.259 | 0.001 | ||||
| Regulation of transcription | ||||||||
| Hoxa5 | homeo box A5 | NM_010453 | 3.701 | 0.000 | ||||
| Nfkbiz | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, ζ | NM_030612 | 2.657 | 0.002 | ||||
| Sox5† | SRY-box containing gene 5 | AK081802 | 2.279; 2.915 | 0.005; 0.006 | ||||
| Foxc1 | forkhead box C1 | NM_008592 | 2.256 | 0.003 | ||||
| Gata2 | GATA binding protein 2 | NM_008090 | 2.250 | 0.001 | ||||
| Hoxa2† | homeo box A2 | NM_010451 | 2.083; 2.150 | 0.003; 0.001 | ||||
| Hoxa7 | homeo box A7 | NM_010455 | 2.027 | 0.021 | ||||
| Msx1 | homeo box, msh-like 1 | NM_010835 | −6.173 | 0.001 | ||||
| Tcfap2b† | transcription factor AP-2β | NM_001025305 | −5.434; −3.367; −3.289 | 0.000; 0.012; 0.008 | ||||
| Tbx4 | T-box 4 | NM_011536 | −2.264 | 0.006 | ||||
| Irf7 | interferon regulatory factor 7 | NM_016850 | −2.220 | 0.041 | ||||
| Parp14 | poly(ADP-ribose) polymerase family, member 14 | NM_001039530 | −2.206 | 0.038 | ||||
| Hoxd8 | homeo box D8 | XM_355338 | −2.192 | 0.001 | ||||
| Tcfap2a | transcription factor AP-2, α | NM_011547 | −2.150 | 0.003 | ||||
| Signal transduction | ||||||||
| Tgm2 | transglutaminase 2, C polypeptide | NM_009373 | 2.344 | 0.001 | ||||
| Pde1a | phosphodiesterase 1A, calmodulin-dependent | NM_016744 | 2.224 | 0.002 | ||||
| Ccl9 | chemokine (C-C motif) ligand 9 | NM_011338 | 2.163 | 0.000 | ||||
| P2ry14† | purinergic receptor P2Y, G protein-coupled, 14 | NM_133200 | 2.070; 2.282 | 0.000; 0.001 | ||||
| Gpr49 | leucine rich repeat containing G protein-coupled receptor 5 | NM_010195 | −6.250 | 0.006 | ||||
| Cxcl15 | chemokine (C-X-C motif) ligand 15 | NM_011339 | −3.257 | 0.014 | ||||
| Tbxa2r† | thromboxane A2 receptor | NM_009325 | −3.058; −2.890 | 0.004; 0.004 | ||||
Genes shown are those that could be functionally categorized, based on their most prominently recognized functions. Of these, 24 and 28 genes were found to be up- and downregulated, respectively.
BLAST analyses indicate that this probe can detect α-actin-like EST sequences in nonmuscle tissues, as skin, mammary gland, and bone. No significant alterations in the expression levels of other actin isoforms (Acta2, Actb, Actc1, Actg1, Actg2) were detected.
Genes identified by multiple probes. The corresponding fold changes and P values are listed for each probe.
Genes included in more than one category. For example, Slit3 is an extracellular matrix protein as well as a cell motility-related protein; Tnn is implicated not only in the regulation of cell motility but also proliferation as well as in and cell adhesion. Moesin has been identified as an upregulated gene in the motility aspect of the invasion signature in cancer cells (12).