Table 2.

Gene expression differences validated by real-time PCR

Gene Name	Gene Symbol	Regulated in Hibernating Bear	Tissue	Array P Value	Real-time PCR P Value
Genes Selected for Validation of Microarray Results
Elongation factor 1 delta	Eef1d	up	liver	0.005	0.001
40S ribosomal protein S24	Rps24	up	liver	0.023	0.027
60S ribosomal protein L36a	Rpl36a	up	liver	0.005	0.006
60S ribosomal protein L18	Rpl18	up	liver	0.027	0.011
60S ribosomal protein L23	Rpl23	up	liver	0.005	0.000
RNA-binding motif protein 3	Rbm3	up	liver	0.005	0.000
Ribosomal protein L27a-like	Rpl27a	up	liver	0.005	0.018
Ribosomal protein L7a	Rpl7a	up	liver	0.005	0.006
Translation elongation factor eEF-1 beta chain	Eef1b2	up	liver	0.013	0.024
Eukaryotic translation initiation factor	Eif2 s2	up	liver	0.005	0.200
60S ribosomal protein L9	Rpl9	up	liver	0.005	0.001
Phosphoenolpyruvate carboxykinase	Pck1	up	liver	0.005	0.000
Proteasome subunit beta type 6 precursor	Psmb6	up	liver	0.021	0.040
Elongation of very long chain fatty acids protein	Elovl2	up	liver	0.005	0.000
Mitochondrial aldehyde dehydrogenase 2	Aldh2	down	liver	0.008	0.008
Phosphoglucomutase 2	Pgm	down	liver	0.007	0.002
Carbonyl reductase [NADPH] 1	Cbr1	down	liver	0.034	0.046
Ornithine aminotransferase	Oat	down	liver	0.000	0.000
Carbamoyl-phosphate synthetase 1	Cps1	down	liver	0.049	0.000
40S ribosomal protein S25	Rps25	up	muscle	0.008	0.017
Cold shock domain-containing protein E1	Csde1	up	muscle	0.030	0.019
Fau protein	Fau	up	muscle	0.035	0.024
60S ribosomal protein L26	Rpl26	up	muscle	0.018	0.009
H3 histone	H3f3a	up	muscle	0.026	0.053
Myosin, light polypeptide 3	Myl3	down	muscle	0.000	0.023
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2	Atp2a2	down	muscle	0.000	0.970
Troponin T, slow skeletal muscle	Tnnt1	down	muscle	0.000	0.020
L-lactate dehydrogenase	Ldhb	down	muscle	0.020	0.580
Additional Genes Selected on the Basis of GO Analysis
40S ribosomal protein S12	Rps12	up	liver	ns	0.011
40S ribosomal protein S29	Rps29	up	liver	ns	0.001
40S ribosomal protein S10	Rps10	up	liver	0.058	0.000
Argininosuccinate synthetase	Ass	down	liver	0.088	0.089
Glucokinase	Gck	down	liver	na	0.067

Significance levels for genes nonsupported by real-time PCR are in boldface.