Figure 2.

Effects of exogenous PGE₂ on TRAP⁺ MNC formation in bone marrow cultures treated with 1,25-D and PTH. Marrow was pooled from several PGHS-2^+/+ mice (white bars) or PGHS-2^–/– mice (black bars) and was cultured for 7 days with vehicle (Con), 1,25-D (10 nM), or PTH (10 nM), with and without PGE₂ (1 μM). Bars represent mean ± SE for TRAP⁺ MNC formation in 6 wells. ^ASignificant difference from control group; P < 0.01. ^BSignificant difference from comparably treated +/+ genotype; P < 0.01. ^CSignificant effect of addition of PGE₂; P < 0.01.