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. 2000 Mar 15;105(6):823–832. doi: 10.1172/JCI8195

Figure 9.

Figure 9

Effects of GM-CSF and a blocking antibody to GM-CSF on TRAP+ MNC formation in RANKL- and M-CSF–stimulated spleen cell cultures. Spleen cells from PGHS-2+/+ (white bars) and PGHS-2–/– (black bars) mice were cultured for 6 days with RANKL (10 ng/mL) and M-CSF (10 ng/mL). Cultures were also treated with vehicle (Control), murine GM-CSF (1 ng/mL), murine polyclonal antibody to GM-CSF (1 μg/mL), or PGE2 (1 μM). Bars represent mean ± SE for quadruplicate cultures. ASignificant effect of –/– genotype; P < 0.01. BSignificant effect of GM-CSF; P < 0.01. CSignificant effect of anti–GM-CSF antibody or PGE2; P < 0.01.