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. 2013 Sep 16;8(9):e74175. doi: 10.1371/journal.pone.0074175

Figure 4. ZFX mRNA and protein expression in the human bronchial epithelial cell line and lung cancer cell lines.

Figure 4

The effect of ectopic miR-144 expression on ZFX protein level in A549 cells. A. Relative mRNA expression levels of ZFX in A549, CRL-5875, HTB-183 and 16HBE14o- cells, mean±SD, n = 3. B. Western-blots of ZFX and GAPDH in above cells. C. Western-blots of ZFX and GAPDH in A549 cells with or without mir-144 over-expression. D. The scheme of the luciferase assay using pMIR-REPORT System to evaluate the direct inhibition of mir-144 on ZFX protein expression. E. The different effects of mir-144 on ZFX 3′-UTR and its mutant, mean±SD, n = 5.Mir-144 can efficiently inhibit the luciferase expression by binding to ZFX 3′-UTR, but has no effect on the luciferase expression when the binding sites were mutated on ZFX 3′-UTR.