Figure 3. Fluorescence during chondrogenic differentiation of ES, C2-24, and C2-27 via EB formation.

(A) Morphology, Alcian blue staining, and fluorescent view. 0 d: during maintenance on feeder cells, 6 d: 1 day after plating of EBs, 18 d: after an additional 12 days of culture, ES-M: ES medium, Diff-M: basal differentiation medium, Jag1: Jag-1 peptide, TD: TD-198946. Scale bars, 50 µm. (B) mRNA levels of chondrocyte-specific collagen genes and Sox genes. Data are expressed as means (bars) ± SDs (error bars) for four wells/group.