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. 2013 Sep 16;8(9):e74083. doi: 10.1371/journal.pone.0074083

Figure 4. BMP7 results in altered M1/M2 balance in BAT and peritoneal macrophages.

Figure 4

4-week-old male C57Bl/6J mice were treated for 4 weeks with BMP7 (33 or 100 µg/kg/day) or saline via a subcutaneously located osmotic minipump at an environmental temperature of 21°C or 28°C while feeding a high-fat diet (45% fat). Expression of the M2 markers Mrc1 (A) and Cd163 (B) and the M1 markers Nos2 (C) and Tnf (D) was measured in BAT by Q-RT-PCR of mice housed at 21°C (left) and 28°C (right). (E) After 4 weeks of treatment, peritoneal macrophages were isolated and markers of M2 and M1 macrophages were measured by Q-RT-PCR of mice housed at 21°C. (F) Bone-marrow derived macrophages were isolated from untreated C57Bl/6J mice and treated ex vivo with BMP7 (8.3 nM) for 24 hrs. The expression of markers for M2 and M1 macrophages was measured via Q-RT-PCR. Values are means+SEM (n = 9 for in vivo and n = 3 for in vitro) and expression of genes was corrected for the housekeeping genes β2-microglobulin and 36b4. *P<0.05 compared to the control group.