Figure 1. NDRG1 expression in trophoblast cell lines in response to hypoxia or CoCl₂.

NDRG1 (upper panels) and tubulin (loading control, lower panels) were detected using Western immunoblot. (A) NDRG1 expression in trophoblasts and trophoblast-derived choriocarcinoma cell lines, as well as in other cell types under standard conditions. (B) NDRG1 expression in JEG-3 cells cultured in standard conditions, 24 h of hypoxia (<1% O₂) or the hypoxia mimetic CoCl₂ (200 µM). The expression of HIF-1α (middle panel) confirmed the effect of CoCl₂ or hypoxia. (C) A time course and concentration-dependent stimulation of NDRG1 expression in JEG-3 cells exposed to the hypoxia mimetic agent CoCl₂. Data are representative of at least three independent experiments.