Figure 2. RM7 mutant presents a single gene mutation at locus AN8741.2.

A) Diagram showing the genomic insert present in the complementation vector from library pRG3-AMA1-NOT1. The insert contains two ORFs corresponding to AN8741.2 and adjacent AN8740.2. The coding region at AN8741.2 locus encodes a putative C₂H₂ zinc finger domain transcription factor. The revertant mutation in RM7 occurred at AN8741.2, designated as mtfA gene. B) Amino acid alignment of A. nidulans MtfA (Ani) and putative orthologs in A. terreus (Ate), A. flavus (Afl), A. clavatus (Acl) and A. fumigatus (Afu). ClustalW (http://www.ebi.ac.uk/Tools/clustalw2/index.htm) land boxshade (http://www.ch.embnet.org/software/BOX_form.html) multiple sequence alignment software programs were utilized in this analysis. The mutation occurred at the codon corresponding to the first methionine. The two conserved zinc finger domains are indicated in A) and B).