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. 2013 Sep 16;8(9):e74122. doi: 10.1371/journal.pone.0074122

Figure 7. mtfA is necessary for normal expression of terrequinone genes.

Figure 7

A) Wild type (WT) veA+ control (TRV50.2), ΔmtfA (TRVpΔmftA) and ΔmtfA-com complementation strain (TRVΔmtfA-com) were inoculated in liquid GMM. Mycelia were collected at 48 h and 72 h after inoculation for RNA extraction. Cultures were grown in a shaker incubator at 37°C at 250 rpm. Expression of tdiA and tdiB was analyzed by Northern blot. 18S rRNA serves as loading control. B) Isogenic wild type (WT) veA+ control (TRV50.1) and over-expression (OE) mtfA strain (TRV60) were inoculated in liquid GMM and grown for 16 h. After that, equal amounts of mycelium were transferred to TMM and further incubated for 24 h and 48 h. tdiA and tdiB expression was analyzed as in (B). Densitometries were carried out with the Scion Image Beta 4.03 software. Asterisk indicates not detected.