Table 4. Activity of CT by using 0.1-ala-ala-pro-phe-p-nitroanilide (SAPNA) in various media in the presence and absence of APPJ exposure for 5 min.

Sample	A410 nm/min	CT Concentration(mg/ml)	CT in ReactionMixture (mg/10 µl)	Specific Activity(unit/mg)	Relative SpecificActivity of CT (%)
Buffer	0.6647	0.2553	0.0025	22.4839±0.0081	100.00
Buffer+APPJ	0.0092	0.3064	0.0031	0.2593±0.0026	1
TEAP	1.1085	0.6765	0.0067	14.1493±0.0078	62
TEAP+APPJ	0.965	0.6340	0.0063	13.1443±0.0072	58
TEAS	0.7102	0.5915	0.0059	10.3696±0.0063	46
TEAS+APPJ	0.5679	0.5531	0.0055	8.8659±0.0013	39
[Bmim][Cl]	0.3245	0.5617	0.0056	4.9893±0.0092	22
[Bmim][Cl]+APPJ	0.1759	0.5574	0.0056	2.7252±0.0083	12
[Mim][Cl]	0.5120	1.0425	0.0104	4.2413±0.0058	18
[Mim][Cl]+APPJ	0.0392	0.7659	0.0076	0.4419±0.0074	2