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Voltage-gated Na+ channel blocker tetrodotoxin (TTX) suppressed ATP release from type II cells. A: representative records from a type II cell by application of cyclohexamide (Chx, 1 mM). TTX (−), TTX-free; TTX (+), 50 nM TTX in the bath and the recording electrode solutions. B: effects of TTX (50 nM) on ATP release from type II cells. TTX (−), n = 7; TTX (+), n = 5; **P < 0.01. ATP release was significantly suppressed by TTX (P = 0.002, t-test).
