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. 2013 Sep 16;8(9):e74749. doi: 10.1371/journal.pone.0074749

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© 2013 Suzuki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Ing3 mRNA was significantly decreased in the fully grown GV oocytes injected with siIng3 (*p<0.05). (B) ING3 protein levels, after normalization to α-tubulin, were decreased in fully grown GV oocytes injected with siIng3. (C) Abnormal cell division was observed in several siIng3-injected oocytes at the MII stage (arrows). (D) In siIng3-injected oocytes, the rate of symmetric division was significantly increased as compared to that observed in siControl-injected oocytes (*p<0.05). (E) Maturation rates were not different between siIng3- and siControl-injected oocytes.