Figure 2. Effect of lentiviral-mediated knock-down of AR on hepatic lipoperoxide content, CYP2E1 expression in db/db mice and over-expression of AR on CYP2E1 expression and ROS levels in AML12 cells.

Hepatic lipoperoxide content (A), CYP2E1 mRNA (B) and protein (C) expression were determined in mice fed the control diet, MCD diet, or MCD diet treated with lentiviruses carrying shRNA against AR for 8 weeks (n = 4). CYP2E1 protein expression (D) and ROS levels (E) were assayed in AML12 cells transfected with pFLAG-mAR for 36 hours (n = 3). Values are expressed as the mean ± SEM. ***, P<0.001; **, P<0.01; *, P<0.05.