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. 2013 Sep 16;8(9):e71100. doi: 10.1371/journal.pone.0071100

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© 2013 Alcalay et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) and (b) RT-qPCR of Popdc1-3 and LacZ mRNAs from hearts isolated upon heart removal (Basal), at the end of stabilization (Stab) and at 90 min reperfusion (I/R). N = 5/group. AU, arbitrary units, Mean ± SEM; *P<0.05 compared to Basal. (c) Western blots of Popdc1 in WT hearts (Popdc1, ∼68 kDa; Actin, ∼42 kDa). (d) Cytochemical staining of LacZ activity (blue nuclei). Note, a higher intensity in subendocardial cells (white asterisk). Eosin counterstaining illustrates the general morphology. Images were captured at X50 and X100 magnification, as specified.